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adenosine kinase inhibitor abt 702  (MedChemExpress)


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    MedChemExpress adenosine kinase inhibitor abt 702
    Effects of inhibitors of adenosine metabolism on the utilization of adenosine for ATP restoration in glucose-fed astrocytes. Astrocyte cultures had been preincubated for 60 min in glucose-free IB with 1 µM of BAM15 to lower the cellular ATP content before the cells were incubated in glucose (5 mM)-containing IB without or with 30 µM adenosine in the absence or the presence of the adenosine kinase <t>inhibitor</t> <t>ABT-702</t> (ABT; 10 µM), the adenosine deaminase inhibitor DCF (1 µM) and/or the purine nucleoside phosphorylase inhibitor forodesine (Foro; 10 µM). After 60 min, the cellular ATP content ( a ) and the extracellular LDH activity ( b ) were determined. The data shown are means ± SD of values obtained in three experiments performed on independently prepared cultures. The average initial ATP content of the cultures (36.8 ± 1.6 nmol/mg) is indicated by the black dotted line in panel a. The protein content of the cultures was 130 ± 12 µg/well and the initial cellular LDH activity 119 ± 5 nmol/(min × well). The significance of differences (ANOVA) compared with the data obtained for the respective control incubation (absence of all inhibitors) is indicated by *** p < 0.001. The significance of differences (t-test) between data for adenosine-free and the respective adenosine-containing incubations is indicated by # p < 0.05, ## p < 0.01and ### p < 0.001
    Adenosine Kinase Inhibitor Abt 702, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/adenosine+kinase+inhibitor+abt+702/ADK%2FAdenosine+Kinase%2C+Human/pmc11569012-59-1-16
    Average 92 stars, based on 1 article reviews
    adenosine kinase inhibitor abt 702 - by Bioz Stars, 2026-11
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    1) Product Images from "ATP Restoration by ATP-Deprived Cultured Primary Astrocytes"

    Article Title: ATP Restoration by ATP-Deprived Cultured Primary Astrocytes

    Journal: Neurochemical Research

    doi: 10.1007/s11064-024-04276-9

    Effects of inhibitors of adenosine metabolism on the utilization of adenosine for ATP restoration in glucose-fed astrocytes. Astrocyte cultures had been preincubated for 60 min in glucose-free IB with 1 µM of BAM15 to lower the cellular ATP content before the cells were incubated in glucose (5 mM)-containing IB without or with 30 µM adenosine in the absence or the presence of the adenosine kinase inhibitor ABT-702 (ABT; 10 µM), the adenosine deaminase inhibitor DCF (1 µM) and/or the purine nucleoside phosphorylase inhibitor forodesine (Foro; 10 µM). After 60 min, the cellular ATP content ( a ) and the extracellular LDH activity ( b ) were determined. The data shown are means ± SD of values obtained in three experiments performed on independently prepared cultures. The average initial ATP content of the cultures (36.8 ± 1.6 nmol/mg) is indicated by the black dotted line in panel a. The protein content of the cultures was 130 ± 12 µg/well and the initial cellular LDH activity 119 ± 5 nmol/(min × well). The significance of differences (ANOVA) compared with the data obtained for the respective control incubation (absence of all inhibitors) is indicated by *** p < 0.001. The significance of differences (t-test) between data for adenosine-free and the respective adenosine-containing incubations is indicated by # p < 0.05, ## p < 0.01and ### p < 0.001
    Figure Legend Snippet: Effects of inhibitors of adenosine metabolism on the utilization of adenosine for ATP restoration in glucose-fed astrocytes. Astrocyte cultures had been preincubated for 60 min in glucose-free IB with 1 µM of BAM15 to lower the cellular ATP content before the cells were incubated in glucose (5 mM)-containing IB without or with 30 µM adenosine in the absence or the presence of the adenosine kinase inhibitor ABT-702 (ABT; 10 µM), the adenosine deaminase inhibitor DCF (1 µM) and/or the purine nucleoside phosphorylase inhibitor forodesine (Foro; 10 µM). After 60 min, the cellular ATP content ( a ) and the extracellular LDH activity ( b ) were determined. The data shown are means ± SD of values obtained in three experiments performed on independently prepared cultures. The average initial ATP content of the cultures (36.8 ± 1.6 nmol/mg) is indicated by the black dotted line in panel a. The protein content of the cultures was 130 ± 12 µg/well and the initial cellular LDH activity 119 ± 5 nmol/(min × well). The significance of differences (ANOVA) compared with the data obtained for the respective control incubation (absence of all inhibitors) is indicated by *** p < 0.001. The significance of differences (t-test) between data for adenosine-free and the respective adenosine-containing incubations is indicated by # p < 0.05, ## p < 0.01and ### p < 0.001

    Techniques Used: Incubation, Activity Assay, Control

    Prevention of the exclusive utilization of adenosine for ATP restoration by inhibitors of adenosine metabolism. Astrocyte cultures had been preincubated for 60 min in glucose-free IB with 1 µM of BAM15 to lower the cellular ATP content before the cells were incubated in glucose-free IB with 100 µM adenosine in the absence or the presence of the adenosine kinase inhibitor ABT-702 (ABT; 10 µM), the adenosine deaminase inhibitor DCF (1 µM) and/or the purine nucleoside phosphorylase inhibitor forodesine (Foro; 10 µM). After 60 min, the cellular ATP content ( a ) and the extracellular LDH activity ( b ) were determined. The data shown are means ± SD of values obtained in three experiments performed on independently prepared cultures. The average initial ATP content of the cultures (37.3 ± 2.4 nmol/mg) is indicated by the black dotted line in panel a. The protein content of the cultures was 136 ± 22 µg/well and the initial cellular LDH activity was 163 ± 59 nmol/(min × well). The significance of differences (ANOVA) compared with the data obtained for the control incubation (absence of all inhibitors) is indicated by *** p < 0.001. The significance of differences (ANOVA) compared with the data obtained after the 60 min pre-incubation (8.1 ± 4.4 nmol/mg; indicated by the black dashed lines in panel a) is indicated by + p < 0.05 and +++ p < 0.001
    Figure Legend Snippet: Prevention of the exclusive utilization of adenosine for ATP restoration by inhibitors of adenosine metabolism. Astrocyte cultures had been preincubated for 60 min in glucose-free IB with 1 µM of BAM15 to lower the cellular ATP content before the cells were incubated in glucose-free IB with 100 µM adenosine in the absence or the presence of the adenosine kinase inhibitor ABT-702 (ABT; 10 µM), the adenosine deaminase inhibitor DCF (1 µM) and/or the purine nucleoside phosphorylase inhibitor forodesine (Foro; 10 µM). After 60 min, the cellular ATP content ( a ) and the extracellular LDH activity ( b ) were determined. The data shown are means ± SD of values obtained in three experiments performed on independently prepared cultures. The average initial ATP content of the cultures (37.3 ± 2.4 nmol/mg) is indicated by the black dotted line in panel a. The protein content of the cultures was 136 ± 22 µg/well and the initial cellular LDH activity was 163 ± 59 nmol/(min × well). The significance of differences (ANOVA) compared with the data obtained for the control incubation (absence of all inhibitors) is indicated by *** p < 0.001. The significance of differences (ANOVA) compared with the data obtained after the 60 min pre-incubation (8.1 ± 4.4 nmol/mg; indicated by the black dashed lines in panel a) is indicated by + p < 0.05 and +++ p < 0.001

    Techniques Used: Incubation, Activity Assay, Control

    Related Articles

    Incubation:

    Article Title: ATP Restoration by ATP-Deprived Cultured Primary Astrocytes
    Article Snippet: Nicotinamide was from Fluka (Steinheim, Germany).Nicotinamide was from Fluka (Steinheim, Germany).. The adenosine kinase inhibitor ABT-702 and the purine nucleoside phosphorylase inhibitor forodesine hydrochloride were obtained from MedChemExpress (Monmouth Junction, NJ, USA).. The Cell Titer Glo ® 2.0 ATP Assay Kit was from Promega (Walldorf, Germany) and UK5099 from Merck (Darmstadt, Germany).The Cell Titer Glo ® 2.0 ATP Assay Kit was from Promega (Walldorf, Germany) and UK5099 from Merck (Darmstadt, Germany).

    Activity Assay:

    Article Title: ATP Restoration by ATP-Deprived Cultured Primary Astrocytes
    Article Snippet: Nicotinamide was from Fluka (Steinheim, Germany).Nicotinamide was from Fluka (Steinheim, Germany).. The adenosine kinase inhibitor ABT-702 and the purine nucleoside phosphorylase inhibitor forodesine hydrochloride were obtained from MedChemExpress (Monmouth Junction, NJ, USA).. The Cell Titer Glo ® 2.0 ATP Assay Kit was from Promega (Walldorf, Germany) and UK5099 from Merck (Darmstadt, Germany).The Cell Titer Glo ® 2.0 ATP Assay Kit was from Promega (Walldorf, Germany) and UK5099 from Merck (Darmstadt, Germany).

    Control:

    Article Title: ATP Restoration by ATP-Deprived Cultured Primary Astrocytes
    Article Snippet: Nicotinamide was from Fluka (Steinheim, Germany).Nicotinamide was from Fluka (Steinheim, Germany).. The adenosine kinase inhibitor ABT-702 and the purine nucleoside phosphorylase inhibitor forodesine hydrochloride were obtained from MedChemExpress (Monmouth Junction, NJ, USA).. The Cell Titer Glo ® 2.0 ATP Assay Kit was from Promega (Walldorf, Germany) and UK5099 from Merck (Darmstadt, Germany).The Cell Titer Glo ® 2.0 ATP Assay Kit was from Promega (Walldorf, Germany) and UK5099 from Merck (Darmstadt, Germany).



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    Effects of inhibitors of adenosine metabolism on the utilization of adenosine for ATP restoration in glucose-fed astrocytes. Astrocyte cultures had been preincubated for 60 min in glucose-free IB with 1 µM of BAM15 to lower the cellular ATP content before the cells were incubated in glucose (5 mM)-containing IB without or with 30 µM adenosine in the absence or the presence of the adenosine kinase <t>inhibitor</t> <t>ABT-702</t> (ABT; 10 µM), the adenosine deaminase inhibitor DCF (1 µM) and/or the purine nucleoside phosphorylase inhibitor forodesine (Foro; 10 µM). After 60 min, the cellular ATP content ( a ) and the extracellular LDH activity ( b ) were determined. The data shown are means ± SD of values obtained in three experiments performed on independently prepared cultures. The average initial ATP content of the cultures (36.8 ± 1.6 nmol/mg) is indicated by the black dotted line in panel a. The protein content of the cultures was 130 ± 12 µg/well and the initial cellular LDH activity 119 ± 5 nmol/(min × well). The significance of differences (ANOVA) compared with the data obtained for the respective control incubation (absence of all inhibitors) is indicated by *** p < 0.001. The significance of differences (t-test) between data for adenosine-free and the respective adenosine-containing incubations is indicated by # p < 0.05, ## p < 0.01and ### p < 0.001
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    Effects of inhibitors of adenosine metabolism on the utilization of adenosine for ATP restoration in glucose-fed astrocytes. Astrocyte cultures had been preincubated for 60 min in glucose-free IB with 1 µM of BAM15 to lower the cellular ATP content before the cells were incubated in glucose (5 mM)-containing IB without or with 30 µM adenosine in the absence or the presence of the adenosine kinase inhibitor ABT-702 (ABT; 10 µM), the adenosine deaminase inhibitor DCF (1 µM) and/or the purine nucleoside phosphorylase inhibitor forodesine (Foro; 10 µM). After 60 min, the cellular ATP content ( a ) and the extracellular LDH activity ( b ) were determined. The data shown are means ± SD of values obtained in three experiments performed on independently prepared cultures. The average initial ATP content of the cultures (36.8 ± 1.6 nmol/mg) is indicated by the black dotted line in panel a. The protein content of the cultures was 130 ± 12 µg/well and the initial cellular LDH activity 119 ± 5 nmol/(min × well). The significance of differences (ANOVA) compared with the data obtained for the respective control incubation (absence of all inhibitors) is indicated by *** p < 0.001. The significance of differences (t-test) between data for adenosine-free and the respective adenosine-containing incubations is indicated by # p < 0.05, ## p < 0.01and ### p < 0.001

    Journal: Neurochemical Research

    Article Title: ATP Restoration by ATP-Deprived Cultured Primary Astrocytes

    doi: 10.1007/s11064-024-04276-9

    Figure Lengend Snippet: Effects of inhibitors of adenosine metabolism on the utilization of adenosine for ATP restoration in glucose-fed astrocytes. Astrocyte cultures had been preincubated for 60 min in glucose-free IB with 1 µM of BAM15 to lower the cellular ATP content before the cells were incubated in glucose (5 mM)-containing IB without or with 30 µM adenosine in the absence or the presence of the adenosine kinase inhibitor ABT-702 (ABT; 10 µM), the adenosine deaminase inhibitor DCF (1 µM) and/or the purine nucleoside phosphorylase inhibitor forodesine (Foro; 10 µM). After 60 min, the cellular ATP content ( a ) and the extracellular LDH activity ( b ) were determined. The data shown are means ± SD of values obtained in three experiments performed on independently prepared cultures. The average initial ATP content of the cultures (36.8 ± 1.6 nmol/mg) is indicated by the black dotted line in panel a. The protein content of the cultures was 130 ± 12 µg/well and the initial cellular LDH activity 119 ± 5 nmol/(min × well). The significance of differences (ANOVA) compared with the data obtained for the respective control incubation (absence of all inhibitors) is indicated by *** p < 0.001. The significance of differences (t-test) between data for adenosine-free and the respective adenosine-containing incubations is indicated by # p < 0.05, ## p < 0.01and ### p < 0.001

    Article Snippet: The adenosine kinase inhibitor ABT-702 and the purine nucleoside phosphorylase inhibitor forodesine hydrochloride were obtained from MedChemExpress (Monmouth Junction, NJ, USA).

    Techniques: Incubation, Activity Assay, Control

    Prevention of the exclusive utilization of adenosine for ATP restoration by inhibitors of adenosine metabolism. Astrocyte cultures had been preincubated for 60 min in glucose-free IB with 1 µM of BAM15 to lower the cellular ATP content before the cells were incubated in glucose-free IB with 100 µM adenosine in the absence or the presence of the adenosine kinase inhibitor ABT-702 (ABT; 10 µM), the adenosine deaminase inhibitor DCF (1 µM) and/or the purine nucleoside phosphorylase inhibitor forodesine (Foro; 10 µM). After 60 min, the cellular ATP content ( a ) and the extracellular LDH activity ( b ) were determined. The data shown are means ± SD of values obtained in three experiments performed on independently prepared cultures. The average initial ATP content of the cultures (37.3 ± 2.4 nmol/mg) is indicated by the black dotted line in panel a. The protein content of the cultures was 136 ± 22 µg/well and the initial cellular LDH activity was 163 ± 59 nmol/(min × well). The significance of differences (ANOVA) compared with the data obtained for the control incubation (absence of all inhibitors) is indicated by *** p < 0.001. The significance of differences (ANOVA) compared with the data obtained after the 60 min pre-incubation (8.1 ± 4.4 nmol/mg; indicated by the black dashed lines in panel a) is indicated by + p < 0.05 and +++ p < 0.001

    Journal: Neurochemical Research

    Article Title: ATP Restoration by ATP-Deprived Cultured Primary Astrocytes

    doi: 10.1007/s11064-024-04276-9

    Figure Lengend Snippet: Prevention of the exclusive utilization of adenosine for ATP restoration by inhibitors of adenosine metabolism. Astrocyte cultures had been preincubated for 60 min in glucose-free IB with 1 µM of BAM15 to lower the cellular ATP content before the cells were incubated in glucose-free IB with 100 µM adenosine in the absence or the presence of the adenosine kinase inhibitor ABT-702 (ABT; 10 µM), the adenosine deaminase inhibitor DCF (1 µM) and/or the purine nucleoside phosphorylase inhibitor forodesine (Foro; 10 µM). After 60 min, the cellular ATP content ( a ) and the extracellular LDH activity ( b ) were determined. The data shown are means ± SD of values obtained in three experiments performed on independently prepared cultures. The average initial ATP content of the cultures (37.3 ± 2.4 nmol/mg) is indicated by the black dotted line in panel a. The protein content of the cultures was 136 ± 22 µg/well and the initial cellular LDH activity was 163 ± 59 nmol/(min × well). The significance of differences (ANOVA) compared with the data obtained for the control incubation (absence of all inhibitors) is indicated by *** p < 0.001. The significance of differences (ANOVA) compared with the data obtained after the 60 min pre-incubation (8.1 ± 4.4 nmol/mg; indicated by the black dashed lines in panel a) is indicated by + p < 0.05 and +++ p < 0.001

    Article Snippet: The adenosine kinase inhibitor ABT-702 and the purine nucleoside phosphorylase inhibitor forodesine hydrochloride were obtained from MedChemExpress (Monmouth Junction, NJ, USA).

    Techniques: Incubation, Activity Assay, Control